Abstract: 【Objective】 To understand Dm86 gene and its coding protein of Bm86 homologue，provide the basis for anti-Dermacenter marginatus vaccine candidate antigen. 【Method】 this experiment amplified the Dm86 gene using the cDNA of fulminated female ticks，truncated genes to construct recombinant plasmids，SDS-PAGE identified after IPTG induction，purified protein and prepared polyclonal antibodies for Western Blotting identification. 【Result】 The target fragment of 1，773 bp was obtained by PCR amplification. According to the analysis of biological characteristics，Dm86 protein was presumed to be composed of 591 amino acids，with molecular weight of 64857.81 and theoretical isoelectric point of 6.78. It was acidic，had unstable hydrophilicity and multiple B cell epitopes. Fluorescence quantitative PCR results showed that Dm86 gene was expressed to different degrees in different stages of ticks，and the expression level was higher in ticks at satiety stage. High antigenicity truncated gene building proteins. SDS-PAGE results showed that specific bands appeared around 39 kDa and expressed as an inclusion body. The titer of polyclonal antibody was 1∶25600，Western blot results show that，the recombinant protein can react with polyclonal antibody to produce specific bands. 【Conclusion】 Recombinant protein Dm86 has certain reactivity and immunogenicity，which can lay the foundation for the study of candidate antigens of vaccine against D. marginatus.