Abstract: 【Objective】 To study the diversity of gut bacteria from adults of field-collected Dendrolimus kikuchii， which results could lay a foundation for the prevention and control of D. kikuchii and the protection of Pinus yunnanensis and other trees. 【Method】 The total gut DNA was extracted from male moths and female moths of D. kikuchii respectively.The Illumina NovaSeq （PE250） high-throughput platform was used to sequence and analysis the V3~V4 regions of the gut bacterial 16S rDNA gene， and the gut bacterial community structure and diversity from male adults and female adults of D. kikuchii were analyzed. 【Results】 A total of 1278 OTUs from adults of D. kikuchii， including 28 phyla， 75 classes， 173 orders， 296 families and 550 genera. The results showed that the dominant bacteria in adults of D. kikuchii gut at the phyla level was Proteobacteria（88.27% for female and 88.17% for male）， those at the family level， the dominant bacteria of male moths were Sphingomonadaceae（49.16%） and Comamonadaceae（19.09%）， and the dominant bacteria of female moths were Erwiniaceae（34.09%） and Comamonadaceae（16.68%）， and those at the genus level， the dominant bacteria of male moths was Sphingomonas（48.09%）， and the dominant bacteria of female moths was Pantoea（31.05%）. The species diversity of gut bacteria in female moths of D. kikuchii was more abundant than those of male moths. The gut bacteria whose abundance showed the most significant difference was Alphaproteobacteria in male moths of D. kikuchii， and Gammaproteobacteria was in female moths of D. kikuchii. 【Conclusion】 The species of gut bacteria are rich from the adults of D. kikuchii.