Abstract: 【Objective】 To determine the content of Z/E isomers in lycopene micelles. 【Method】 HPLC method， The column was YMC Carotenoid S-5（4.6 mm×250 mm）； Mobile phase A：acetonitrile B：methyl tert-butyl ether； Linear gradient elution； Flow rate：1 mL/min； Detection wavelength：472 nm； Column temperature：30℃， Complete the separation and detection of isomers within 45 minutes， the isomers in the lycopene micelles are analyzed and identified through the characteristic peaks and absorption intensity on the ultraviolet-visible absorption spectrum. 【Result】 The linear ranges of Lycopene was 0.22~6.57 μg/mL （r=0.999）， Whose average recoveries were 100.06%（RSD=1.99%）. It shows that the method has strong specificity， good linear relationship， fast， accurate and simple， and is suitable for the determination of isomers in lycopene micelles. 【Conclusion】 Determined that there are all-trans forms in the lycopene micelles：All-E， and three cis isomers：13Z， 9Z， 5Z.with an average relative percentage of 76.06%，10.83%，4.55%，8.56%， respectively.