Abstract: 【Objective】 This study was aim to ascertain the effect on the Hami melon pulp softening and cell wall degradation enzymes with vibration stress during storage. 【Method】 The experiment used the thick-skinned muskmelon Xizhoumi 17 as test material，According to GB/T4857.7-2005 standard，using the low-frequency vibration test bench to carry out the fixed-frequency vibration test on hami melon stacking. Comparing the changes of maximum pressure，pulp hardness and cell wall degrading enzyme between vibration stress and none vibration stress. 【Result】 During the post-harvest storage period at room temperature，the maximum pressure and flesh hardness of 'Xizhoumi 17' Hami melon showed a trend of gradual decline，PG activity，CEL activity and PE activity showed a trend of first increase and then decrease，and β-Gal activity showed a trend of gradual increase. 【Conclusion】 Vibration stress could significantly reduce the maximum pressure and flesh hardness of fruit.In terms of impact on the cell wall degradation enzymes，vibration stress can significantly improve the level of activity expression of pulp CEL，15 days before storage，vibration stress can significantly improve the level of pulp PE activity，the expression，the storage effect is not obvious in the late，late vibration stress can significantly improve the storage pulp PG activity expression level，the vibration stress on pulp β-GAL activity effect is not obvious.