Abstract: 【Objective】 To analyze the genetic diversity of purple sweet potato varieties collected from China, clarify their genetic differences in the hope of providing a theoretical basis for the selection of purple sweet potato genetic breeding parents. 【Methods】 Morphological characters and ISSR fluorescent labeling capillary electrophoresis were carried out to analyze the genetic diversity of varieties of 10 purple sweet potatoes imported from other provinces. 【Results】 The results showed that, based on the cluster map of 20 morphological traits, the tested materials could be divided into two major groups at the Euclidean distance of 9.97. The first major category included Ningzi-1, Shandongzi, Yuzi-2, Xuzi-8, Yuenanzi; the second major category consisted of E-12, Ningzi-4, Yuzi-3, Yuzi-11, Nanzi-018. 6 pairs of primers were used in ISSR molecular marker analysis, the results showed that, the observed alleles (Na) of the purple sweet potato germplasm resources were 2, the average effective allele number (Ne) was 1.9190, and the expected heterozygosity (He) was 0.2375 and Shannon's index (I) was 0.4333. Cluster analysis performed with NTSYSpc 2.1 software demonstrated that when the genetic distance was 0.96, the 10 tested purple sweet potato materials could be divided into two categories. 【Conclusion】 The clustering results of SSR markers and morphological markers of several tested materials were consistent in genetic background and group classification, but the cluster analysis results of the two identification and classification methods also had certain differences. The purple sweet potato resources presented very rich genetic diversity. The combination of morphology and ISSR markers could effectively improve the identification of specific varieties and determine the genetic relationship of germplasm resources objectively and accurately.