Abstract: 【Objective】 To study the classification of the genus: Cleroclytus collaris Jakovlev,1885 and Cleroclytus strigicollis Jakovlev,1900 by combining DNA barcoding technology and morphological characteristics in the hope of providing a research basis for its accurate identification and a reference for the related identification of this genus in the future. 【Methods】 The structure of ovipositor and internal genitalia was described by dissecting the female adult genitalia.Genomic DNA was extracted from the head and throx of the adult, and mitochondrial cytochrome oxidase I (COI) was amplified by PCR with universal primers to construct the DNA barcodes. 【Result】 The results of morphology and genitalia structure showed that there was no significant morphological difference between the two species.The results of DNA barcoding showed that the COI gene sequence length was about 670bp.The sequence similarity between Cleroclytus collaris and Cleroclytus strigicollis was 99.60%.The average genetic distance between them was 0.6%, which proved that they were the same species, which was basically consistent with the results of morphological identification. 【Conclusion】 DNA barcoding technology and morphological characteristics are combined to determine the classification of two species of Cleroclytus in Xinjiang, confirming that they are synonyms.