Abstract: 【Objective】 To optimize the optimal process parameters for separation and purification of total flavonoids from Armeniaca sibirica (L.) Lam with macroporous resin. 【Methods】 Ultraviolet spectrophotometry was used to determine the total flavonoids content of Armeniaca sibirica (L.) Lam, and the static adsorption and desorption test was used to investigate the adsorption and desorption capacity of 4 different types of macroporous resins on the total flavonoids of Armeniaca sibirica (L.) Lam. The appropriate macroporous resin was selected and finally the best separation and purification conditions were determined. 【Results】 D101 type macroporous resin had a better specific adsorption capacity and desorption capacity for total flavonoids from Armeniaca sibirica (L.) Lam. The best separation and purification process conditions were D-101 macroporous resin with a diameter-to-height ratio of 1∶6, and the loading volume of the crude extract of Armeniaca sibirica (L.) Lam was the largest: 6.3 BV, whose best loading concentration was 0.3 mg/mL, The loading rate was 1.0 mL/min, the standing time was 2 h, the washing volume was 5 BV, and the washing liquid elution flow rate was 1.5 mL/min. 80% ethanol was used as the elution solvent, whose amount was 2 BV, and the eluent flow rate 0.5 mL/min. 【Conclusion】 D101 type macroporous resin can be used for the separation and purification of total flavonoids in Armeniaca sibirica (L.) Lam under the obtained technological conditions.