Abstract: 【Objective】 To study the accumulation and metabolism of malate in Cabernet Sauvignon grapes under the inverted L shape canopy type. 【Method】 On the basis of the inversed L-VSP trellis, a VSP shape and two kinds of inverted L shape canopy types were set up to detect the changes of organic acids such as malate and the activities of malate metabolism related enzymes during the ripening of Cabernet Sauvignon fruits. 【Result】 Two inverted L shape canopy types reduced the temperature of the fruit-zone microenvironment by 0.8℃ and 1.5℃ compared to the VSP shape canopy type. The inverted L shape canopy type could increased the malic acid and tartaric acid content, with ripe fruit having 0.338 mg/g and 0.365 mg/g of malic acid and 0.774 mg/g and 0.760 mg/g of tartaric acid, respectively. The H₁ canopy with higher leaf height is more conducive to the accumulation of malate than the lower H₂ canopy. The PEPC and NAD-MDH enzyme activities of the three canopy types' fruits showed varying degrees of positive correlation with fruit malate content, and NADP-ME enzyme activity showed varying degrees of negative correlation with fruit malate content. 【Conclusion】 The simplified inversed L shape canopy setting can achieve the purpose of regulating malate accumulation and metabolism in Cabernet Sauvignon grapes, which provides the theoretical basis and empirical reference for the study of the regulation of grape organic acid metabolism.