Abstract:
【Objective】 Years of field trials and germplasm genetic diversity analysis screened out 100 germplasms with excellent drought resistance performance as test materials, combined with field drought resistance identification, the chickpea drought resistance core resource bank was initially constructed with a view to provide basic materials for drought resistant germplasm utilization of chickpea in the later stage.
【Methods】 By comparing random sampling, site priority sampling, deviation sampling strategy and sampling ratio, the variation retention of agronomic traits in each core subset was evaluated by means of mean difference percentage, variance difference percentage, extreme difference coincidence rate and variation rate of coefficient of variation, and the final strategy of constructing drought-resistant core library was selected. In addition to that, the core database of drought resistant germplasm of chickpea was determined by principal component analysis.
【Results】 There was no significant difference in diversity between the core collection constructed with the site-first sampling method at 40% sampling ratio and the original collection. And the coincidence rate of the mean value of each trait of the tested germplasm with the original germplasm was greater than 96.33%, and the coincidence rate of the coefficient of variation was greater than 97.25%, which was better than the other two sampling strategies.The four principal components of principal component analysis contained more than 85% genetic diversity information of each quality trait, and 40 core germplasm resources constructed could represent the genetic diversity of the original germplasm resources.
【Conclusion】 Under the site-priority sampling strategy, the chickpea drought-tolerant core germplasm bank established with a sampling ratio of 40% can represent the genetic diversity of the original germplasm resources.