Abstract: 【Objective】 To explore the effect of different rhizobia inoculation. 【Method】 On the growth and nodulation of the multiple sowing soybean, three rhizobia strains (NW5-3, SN7-2 and SMH12) were used as materials, and three inoculation methods of seed fertilizer, application with drip irrigation and seed dressing were carried out on soybean Xindou 8 in southern Xinjiang, the nodule number, nodule dry weight, plant traits, yield and the yield components were measured. 【Result】 Inoculation of rhizobia could promote root nodulation and growth of soybean, and the SN7-2 performed better than the SMH12 and NW5-3.Among the inoculation methods, the seed dressing treatment of rhizobia was the most suitable, followed by application with dripping water.Among them, the optimal yield of the seed dressing SN7-2 treatment combination reached 3,575.23 kg/hm², seed dressing SMH12 treatment combination yield second reached 3,376.03 kg/hm². 【Conclusion】 Under the experimental conditions, the most suitable match was the combination of seed dressing with rhizobia SN7-2 and SMH-12, followed by drip application with rhizobia SN7-2.