Abstract: 【Objective】 This project aims to study the distribution of functional genes for disease resistance of different wheat varieties (lines) and clarify the in the hope of providing theoretical basis for wheat disease resistance breeding. 【Method】 458 wheat varieties (lines) were detected by KASP through one powdery mildew resistance molecular marker (Pm21), one stripe rust resistance molecular marker (Yr15) and two leaf rust resistance molecular markers (Lr14a and Lr68). 【Result】 There were 2 Pm21 carried lines, 14 Yr15 carried lines, 22 Lr14a carried lines and 48 Lr68 carried lines in 458 wheat cultivars (lines). 【Conclusion】 The distribution frequency of resistance genes in Xinjiang wheat varieties (lines) was about 5% and the 86 lines selected can be used as the parents for resistance breeding of wheat in Xinjiang.