Abstract: 【Objective】 This study aims to compare the differences of mineral elements content between Jinghe wolfberry and Zhongning wolfberry, and to explore the feasibility of identifying the geographical origin of wolfberry based on multiple mineral elements analysis technology, so as to clarify the differences of mineral element fingerprint characteristics between them. 【Methods】 The content of 52 mineral elements in 102 wolfberry samples from Jinghe County, Xinjiang and Zhongning County, Ningxia were determined by inductively coupled plasma mass spectrometry (ICP-MS), and the multiple statistical analysis methods including analysis of variance and orthogonal partial least squares method-discriminant analysis (OPLS-DA) were used for the geographical origin identification. 【Results】 There were significant differences in 26 mineral elements between Jinghe and Zhongning wolfberry (P<0.05). Among the 26 elements, the contents of 16 elements including Mg, Al, P, K, V, Cu, Zn, As, Se, Mo, Ag, Cd, Gd, Er, Pb, and U in Jinghe wolfberry were significantly higher than those in Zhongning, while the contents of 10 elements including Na, Ca, Ni, Rb, Sr, Hf, Ir, Au, Tl and Th in Zhongning were significantly higher than those in Jinghe. Through stepwise discriminant analysis, 14 elements including U, Mo, K, Rb, Au, Ir, Hf, Cu, Tl, Zn, Ca, Th, Mg and Cd were identified as significant element variables. 【Conclusion】 The multi-element discrimination model can be established based on the above 14 elements and the accuracy rates of the geographical origin discrimination of Jinghe and Zhongning wolfberry were 100% and 98.08%, respectively. Jinghe and Zhongning wolfberry have their own unique mineral element fingerprint characteristics, and the use of multiple mineral elements can effectively identify Xinjiang Jinghe wolfberry and Ningxia Zhongning wolfberry.