Abstract: 【Objective】 To analyze content of Staphylococcus aureus in food samples, and evaluate the accuracy and operability of the rapid kit for S.aureus. 【Methods】 Apple chips in bulk and fruit corn in vacuum packaging were sampled, and S.aureus was determined by the method in GB 4789,10-2006 and the rapid kit.S.aureus was taken as the target bacteria, artificial contamination treatments were used, and according to the Technical Specification for Evaluation of Rapid Food Testing Methods, the indexes of the rapid detection kit for S.aureus were evaluated. 【Results】 The highest content of S.aureus in samples of apple chips and fruit corn was 20 and 45 CFU/g, respectively, which were lower than the limit of pathogens in related foods.The sensitivity, specificity, false-negative rate, false-positive rate and relative accuracy of the kit were 93%, 100%, 6.6%, 0, and 96.7%, respectively.Significant difference was not found between the rapid kit and the reference method. 【Conclusion】 The rapid kit is easy to operate and detect S.aureus accurately, which is suitable for fast checks.