Abstract: 【Objective】 To explore the differences in dry matter accumulation, grain filling characteristics and yield among varieties of winter and spring wheat in Northern Xinjiang. 【Methods】 The difference of dry matter accumulation, grain filling characteristics and yield between winter and spring wheat varieties was compared by using for overwinter seeds planting method. 【Results】 The results showed that Xindong 41was 7,812.3 kg/hm², relatively higher than other winter wheat varieties, and that of spring wheat varieties Xinchun6 was 7,935.6 kg/hm² that was higher than other spring wheat varieties. The total dry matter accumulation of winter varieties was between 14,727.8-16,983.26 kg/hm², the total dry matter accumulation of spring varieties was between 13,588.6-17,634.7 kg/hm², and the average dry matter accumulation of winter varieties at maturity (16,140.9 kg/hm²) was 2.09% higher than that of spring varieties (15,804.1 kg/hm²), but the difference between winter and spring varieties was not obvious. The average assimilation to grain distribution, the average distribution rate, and the average granular weight contribution rate of winter wheat varieties were 4,508.46 kg/hm², 57.13%, 78.78% in turn, which was not significantly different from those of spring wheat varieties (4,324.77 kg/hm², 56.36%, 77.71% in order). The average value of the rapid growth duration of winter wheat varieties (16.25 d), the average value of the maximum grouting rate (2.23 g/d), the average grouting rate (1.12 g/d), the winter wheat varieties were 15.70 d, 2.22 g/d, 1.09 g/d in turn, and the difference between winter and spring wheat varieties has not reached a significant level. 【Conclusion】 Combining the above research results, it is believed that Xinchun 6 and Xindong 41 are suitable for the cultivation of wheat varieties for overwinter seeds cultivation in northern Xinjiang.