Abstract: 【 Objective 】 The larvae dung of Tenebrio molitor (L) and Protaetia (Liocola) brevitarsis (Lewis) are both rich in nutrients. This project aims to explore the application potential of the dung from two insects in improving crop yield and quality.【 Method 】 Pepper was chosen as the experimental vegetable, with non-addition of dung group as CK and the plant height, chlorophyll content and vitamin C, total sugar and nitrate content of the fruit were detected by pot culture (10 treatments) with the addition of 0.5%, 1%, 2%, 4% and 8% of Tenebrio molitor larvae dung, and the addition of 2.5%, 5%, 10%, 20%, 40% and 80% Protaetia (Liocola) brevitarsis dung, respectively, there are 10 treatments, and a control treatment without adding insect dung.【 Result 】 All of the plants died in the treatment of 8% dung addition from Tenebrio molitor. After 90 days of pepper planting, highest values of the pepper height, chlorophyll content and yield were achieved in treatment of 1% addition, which were 71.40 cm, 73.39 and 278.23 g/plant, respectively. Moreover, its fruit quality was also the best compared with other treatments, vitamin C, total sugar and nitrate content were 117.15mg / kg, 4.21% and 299.60 mg/kg respectively. When the Protaetia (Liocola) brevitarsis larvae dung was added, the highest plant height with 72.83 cm was observed in treatment of 20% addition. The chlorophyll content of the peppers treated with 80% was the highest, which was 67.14. The highest yield of peppers per plant was 291.45 g in the treatment of 40% addition with significant difference in comparison of the control. The better fruit quality was obtained in the treatment of 20% addition, whose contents of vitamin C, total sugar and nitrate were 144.78 mg/kg, 4.01% and 247.82 mg/kg, respectively. Among them, the contents of vitamin C and total sugar were significantly higher than those of the control.【 Conclusion 】 The addition of 1% Tenebrio molitor larvae dung or 20% Protaetia (Liocola) brevitarsis larvae dung showed the best effect on plant height, chlorophyll content, yield and fruit quality of pepper, and the recommended application range of the two kinds of insect dung are 1%-2% and 20%-40%, respectively.