Abstract: 【 Objective 】 Cynodon dactylon (L.) Pers. is a kind of warm-season turfgrass, which slowly enters the yellowish state after entering autumn, that is, it enters a dormant state. The underground rhizomes and buds of it support its life. The physiological substances in the buds play an important role in responding to dormancy. Understanding the physiological response of dormancy can guide the establishment and management of bermudagrass.【 Method 】 In this paper, the underground buds of bermudagrass from 8 different regions were collected, and physiological indicators such as osmotic adjustment, membrane oxidation, and enzyme activity of Cynodon dactylon (L.) Pers. was measured.【 Result 】 The physiological substances in bermudagrass buds kept evolving with the dormant process (summer - early autumn - late autumn), and the content of free proline and soluble protein in the buds increased continuously, and showed consistency in 8 bermudagrass; The content of SOD and POD reached its peak in the early autumn (dormant start), and then slowly decreased, showing a trend of first increase and then decrease, indicating that the enzyme activity accumulated at the start of dormant; the increase of MDA content indicated that the dormant process was accompanied by oxidation of membrane lipids.【 Conclusion 】 It can be seen from this that physiological substances play an important role in bermudagrass dormancy. A large increase in free proline and soluble protein can be used as a reference index to determine the beginning of dormancy. This study reveals the physiological substance response during bermudagrass dormancy and lays the foundation for further exploring the dormancy mechanism of Cynodon dactylon (L.) Pers.