Abstract: 【 Objective 】 To clarify the temperature effect and its degree on the biological activity of tetrachlorantraniliprole to Helicoverpa armigera, and to explore the influence mechanism of four detoxification enzymes of H. armigera to this temperature effect.【 Methods 】 The authors respectively determined the indoor biological activities of tetrachlorantraniliprole to H.armigera at 15, 20, 25, 30 and 35℃ with the leaf dipping method, and then determined the specific activities of carboxyl esterase (CarE), glutathione S-transferase (GST), multifunctional oxidase (MFO) and UDP-glycosyltransferase (UGT) of H.armigera treated by the sublethal doses of tetrachlorantraniliprole.【 Results 】 Tetrachlorantraniliprole had a significant positive temperature effect on the biological activity to H.armigera. Compared with 15℃, the temperature coefficient increased from +5.52 to +1,257.35. From the study about the mechanism of the four detoxification enzymes of H.armigera to the temperature effect, it was found that the positive temperature effect might be related to GST and MFO. Among them, the activity of GST at lower temperatures changed more significantly, while CarE and UGT were not clearly related to the positive temperature effect on the biological activity of tetrachlorantraniliprole. 【 Conclusion 】 Tetrachlorantraniliprole was an insecticide with an obviously positive temperature coefficient to H. armigera and the biological activity would be best when used at high temperature. The biological activity of tetrachlorantraniliprole against H. armiger may increase when the activity of GST is inhibited at low temperature.