Abstract: 【Objective】 The halophyte Kalidium foliatum is a very salt-tolerant shrub belonging to Chenopodiaceae with strong resistance to salinity.Vacuolar Na⁺/H⁺ antiporter (NHX) is a membrane protein that plays an important role in ionic homeostasis.It maintains intracellular ion and pH balance by regulating the transmembrane transport of ions between cells.To study the salt tolerance function of the vacuolar Na⁺/H⁺ antiporter gene (KfNHX1, AY825250) from K.foliatum, to provide candidate genes for salt tolerance breeding. 【Methods】 The KfNHX1 gene was transferred into Arabidopsis thaliana by the method of Agrobacterium tumefaciens mediated inflorescence transformation; combined with genomic PCR and RT-PCR methods to identify transgene lines with 3:1 segregation ratio; utilize the germination rate, root length and phenotype analysis under salt stress; combine the content of Na⁺ and K⁺ in leaves with an atomic absorption spectrophotometer to infer the salt tolerance. 【Results】 Genomic PCR and RT-PCR analysis were performed on the homozygous transgenic plants by antibiotic screening of 3∶1 to confirm that the KfNHX1 gene was stably integrated into the genome and expressed in Arabidopsis.Further salt tolerant physiological function data show that the transgenic Arabidopsis had higher germination rate and root length than the wild-type under salt stress.The WT and transgenic plants were stressed with 200 mM NaCl for 15 days, the transgenic plants grew well and accumulated high Na⁺ and K⁺ contents in the leaves, but the WT plants were chlorotic and dying.Besides, the transgenic plants have higher germination rate and growth phenotype under exogenous ABA applyment. 【Conclusion】 The results showed that the K.foliatum KfNHX1 can enhance the salt tolerance of transgenic Arabidopsis and has the potential to improvement of plant salt tolerance.