Abstract: 【Objective】 This paper reviews and summarizes the research status and progress of the regulation of tomato miRNAs on its growth and development and response to adversity, so as to provide theoretical and scientific basis for the application of tomato breeding. 【Methods】 Consult the relevant literature at home and abroad, summarize and compare the literature data. 【Result】 MiRNA is a kind of endogenous noncoding microRNA, which widely exists in plants. It is located in the noncoding region of genome and has about 21-25 nucleotides. It regulates target gene expression at the post-transcriptional level by directional degradation of target gene mRNA and inhibition of its translation. The emergence of high-throughput sequencing is conducive to the exponential growth of the number of plant miRNAs, which makes the types and data volume of miRNAs related databases increasingly rich. Recent research results show that many biological processes of tomato are regulated by miRNA, including plant morphology, organ development, growth and development, and response to drought, salt, temperature, and biological stress. 【Conclusion】 MiRNAs play a crucial role in tomato growth and development, and stress response. The research should focus on the regulatory mechanism of miRNAs and their target genes on tomato. In the future, it is expected to change the quality and growth cycle of tomato fruits in a targeted manner, and provide new ideas and methods for tomato resistance to adversity.