Abstract: 【Objective】 To study the effects of combinative application of biochar, organic fertilizer, cake fertilizer, nitrogen, phosphorus and potassium (NPK) fertilizer on sesame yield and soil properties. 【Method】 The field experiment was conducted to study the effects of biochar, organic fertilizer, cake fertilizer and NPK fertilizer on physiological characteristics of sesame, yield, soil physical and chemical property and enzymatic activity. 【Results】 Compared with control, the combinative application of biochar, organic fertilizer, cake fertilizer and NPK fertilizer significantly increased the soil enzyme activity and photosynthetic characteristics of sesame during its flowering stage. The activities of soil saccharase respectively increased by 33.3%, 26.4% and 23.6% compared to the control, the activities of soil urease increased by 47.4%, 35.9% and 29.5%, catalase activity increased by 20.4%, 13.6% and 12.1%, the content of chlorophyll in leaves increased by 10.7% and 9.4% and 8.0%, and the net photosynthetic rate increased by 24.2%, 16.2% and 10.9%. The rapid available contents of nitrogen (N), phosphorus (P) and potassium (K) increased under the combinative application of biochar, organic fertilizer, cake fertilizer and NPK fertilizer treatment. Biochar treatment had a significant effect in reducing soil bulk density and increasing total soil porosity. The combined application of biochar, organic fertilizer, cake fertilizer and NPK fertilizer significantly increased the yield compared with the non-fertilization, and increased the yield by 11.7% -13.3%, 8.8% -8.1%, and 4.4% -8.7% respectively compared with the treatment of NPK fertilizer. 【Conclusion】 It is recommended that 1,500 kg of biochar per hectare or 1,500 kg of organic fertilizer combined with 120 kg of nitrogen(N) fertilizer, 75 kg of phosphorus (P) fertilizer, 75 kg of potassium(K) fertilizer can be applied as the way of fertilization.