Abstract: 【Objective】 To study the drug resistance and virulence of Shiga toxin-producing Escherichia coli from sheep in Xinjing. 【Methods】 Two-month-old sheep's anal swab samples were randomly taken from a large-scale farm. After the samples were enriched in EC broth, the Shiga toxin-producing Escherichia coli was isolated and identified by MAC plate combined with double PCR(stx1, stx2). E. coli O157: H7 was isolated and identified using SMAC plate in combination with double PCR. The susceptibility test of 9 types of 18 drugs was performed using the K-B method. Sensitivity test of 9 types of 18 drugs was conducted by K-B method, and corresponding drug resistance genes were detected by PCR. STEC virulence genes were detected by PCR and tested for "Top Six " and E. coli O157:H7 serotypes. 【Results】 Of the 21 samples, 9 of them contained STEC (42.86%), and one of them contained E. coli O157:H7. Drug susceptibility test showed that only 2 strains were resistant to cefepime and sensitive to other drugs. All isolated strains carry the stx1 virulence gene, and their virulence gene subtypes are mainly stx1a (60%) and stx1c (80%). 9 STECs are not part of the "Top Six" or O157 serotype. 【Conclusion】 From the separation of STEC in the sheep farm, STEC has a high carrying rate, mainly carrying stx1 with weak pathogenicity. The strain isolated from the farm contained the O157:H7 serotype but did not have "Top Six ". The farm's STEC is generally sensitive to current antibiotics, reflecting its good aquaculture environment and less antibiotic use.