Abstract: 【Objective】 To study the relationship between soil microbial biomass carbon (MBC) and microbial biomass nitrogen (MBN) in jujube orchard at different yield levels and soil and plant nutrients in different periods can provide theoretical basis for soil management for high yield of jujube in southern Xinjiang. 【Method】 Zizyphus jujuba orchards with three different yield levels (high, middle and low) in southern Xinjiang were taken as research objects, and through field experiments, the relationship between soil microbial biomass carbon and nitrogen content of different soil layers and plant nitrogen, phosphorus and potassium in Zaoyuan at different yield levels was studied. 【Result】 The results showed that the content of soil available nitrogen and available phosphorus decreased gradually with the increase of soil depths, but available potassium content increased. The correlation between soil (MBC) and soil (MBN) was very significant and the correlation coefficient was 0.836 7. Soil MBC content was significantly and positively correlated with the content of available potassium in the 0 - 20 cm soil layer during exhibition leaf stage of jujube. Meanwhile it was showed significant great positive correlated correlation with available potassium in 20 - 40 cm soil layer during expansion period of jujube. 【Conclusion】 At present, soil MBC, MBN and nutrient content in jujube orchard were maintained at a high level and soil and plant nutrients would have significant impacts on MBC and MBN.