Abstract: 【Objective】 To study the nitrogen absorption and utilization of cotton under drip irrigation with different fertilizer modes. 【Methods】 Four kinds of fertilization methods were set up in the field single-factor randomized block test method. The first type was fertilized in 1/3 time, watered in 1/3 time, fertilized in 1/3 time, and the second type was 1/2 time fertilization, 1/4 time watering, 1/4 time fertilization, 3rd type 1/4 time fertilization, 1/4 time watering, 1/2 time fertilization, 4th type 1/4 time fertilization, 1/2 time watering, 1/4 time fertilization, and they were respectively recorded as W₁, W₂, W₃ and W₄. During the whole process, the total nitrogen content of cotton and the distribution and accumulation of nitrogen in various organs under different drip irrigation conditions were studied. 【Results】 The results showed that (1) the average total nitrogen content of cotton plants under drip irrigation was W₁>W₂>W₃>W₄; (2) the total nitrogen content of cotton under different organs was expressed as leaf > flower bud > boll > stem; (3) The agronomic efficiency of nitrogen fertilizer and the partial productivity of nitrogen fertilizer in drip irrigation cotton under different fertilizer allocation patterns were: W₁>W₃>W₂>W₄. 【Conclusion】 The best NWN mode for nitrogen absorption and utilization and yield of cotton The fertilizer distribution period is W₁ (1/3 time watering, 1/3 time fertilization, 1/3 time watering).