Abstract: 【Objective】 Peroxidase (POD, Peroxidase) activity in wheat grains has an important effect on wheat processing quality and flour color. To understand the peroxidase activity of Xinjiang wheat varieties (lines) and determine the variation types and distribution of related genes, so as to lay a foundation for wheat quality inheritance and improvement in Xinjiang. 【Method】 In this study, 113 Xinjiang wheat cultivars were genotyped by TaPod-3A1/TaPod-3A2 and TaPod-7D1/TaPod-7D6, combined with the results of measuring the POD activity of Xinjiang wheat cultivars the effects of different allelic variation of POD activity-related genes on POD activity of wheat cultivars the validity of the functional markers of TaPod-A1 and TaPod-D1 genes and the frequency of allelic variation of POD related genes in Xinjiang wheat cultivars. 【Result】 In Xinjiang wheat cultivars, the POD activity of TaPod-A1b(2,595.3 U/(g·min)) was significantly higher (P<0.01) than that of TaPod-A1a (2,346.0 U/(g·min)) at TaPod-A1 loci and the distribution frequencies of the two genotypes were 36.3% and 63.7%; the POD activity of TaPod-D1b(2,503.9 U/(g·min)) was significantly higher (P<0.05) than TaPod-D1a genotype(2,376.9 U/(g·min)) at TaPod-D1 loci and the distribution frequencies of the two genotypes were 46.9% and 53.1%. 【Conclusion】 According to the results of this, TaPod-A1a (low POD activity) and TaPod-D1a (low POD activity) the main allelic types; markers of TaPod-A1 and TaPod-D1 can distinguish the POD activity of wheat seeds well. The combination of two site-specific markers can screen out materials with high POD activity more it can more effectively screen materials with high POD activity, increase the frequency of excellent allelic variation of Xinjiang wheat varieties (lines), and promote genetic improvement of Xinjiang wheat quality.