Abstract: 【Objective】 To assess the cross-resistance risk of whitefly Bemisia tabaci (Gennadius) MEAM1 cryptic species to three neonicotinoid insecticides and predict the rate of cross-resistance development in the hope of providing the reference for scientific and rational application of insecticides. MEAM1 Bemisia tabaci (Gennadius) 【Methods】 The cross-resistance strain was developed by group selection, and the realized heritability(h²) of Bemisia tabaci cross-resistance was estimated by Tabashnik's method. 【Results】 Results showed that after being selected by imidacloprid, thiamethoxam and acetamiprid for 9 generations under 50%-70% selection pressure, the cross-resistance ratio of MEAM1 cryptic species to the three neonicotinoids differed from each resistance selected strain. The cross-resistance ratio of imidacloprid selected strain to thiamethoxam and acetamiprid increased by 8.72 -fold and 19.21-fold, respectively, which were low and medium cross-resistance levels and their interactive realized heritability h² were 0.198,1 and 0.285,4, respectively. The cross resistance ratio of acetamiprid strain to imidacloprid and thiamethoxam increased by 10.08-fold and 9.83-fold, respectively, which were low and medium cross-resistance levels and the interactive realized heritability h² were 0.242,3 and 0.128,1, respectively. The cross-resistance ratio of thiamethoxam selected strain to imidacloprid and acetamiprid increased by 3.12-fold and 3.11-fold, respectively, which were in the sensitivity decrease stage and the cross realized heritability h² was 0.142,1 and 0.068,0, respectively. 【Conclusion】 The risk of the imidacloprid selected strain developing cross-resistance to thiamethoxam was less than that of acetamiprid. The risk of the acetamiprid selected strain developing cross-resistance to thiamethoxam was less than that of imidacloprid. The risk of the thiamethoxam selected strain developing cross-resistance to acetamiprid was less than that of imidacloprid.