Abstract: 【Objective】 To broaden the gene pool of cotton germplasm and screen out the germplasm resources suitable for hybrid parents. 【Methods】 the variation of morphological indexes and genetic diversity of 200 cotton germplasms were studied, and the cluster analysis of 200 germplasms carried out. 【Results】 The results showed that the variation range of genetic diversity index (H′) of 21 morphological indexes was 0-1.02, and cluster analysis showed that all materials could be divided into 6 categories, of which,among which 30 materials belongs to the first big category with purplish red color stem. Their seeds are mainly of short velvet grayish brown color and can be used as the breeding resources of color cotton; 20 materials of 170177, whose seeds are of short hair green brown color, can be used as the breeding materials of early maturity, phenol free and high temperature tolerance; 50 were in the sixth category, accounting for a large proportion. 【Conclusion】 The genetic diversity of 200 germplasm resources is abundant, 50 materials of the sixth class can be used as the parent of breeding materials in Xinjiang at present. It is mainly characterized by tower type of plant type, no stem hair, dark green leaf color, medium leaf size, no leaf base spot, limited branch type, yellow corolla, yellow anther, hairy seed pubescence, gray green seed pubescence, pigment gland of seed kernel and white boll color.