Abstract: 【Objective】 The aim of this study is to reveal the salt tolerance characteristics of different genotypes of Gossypium barbadense L. during germination, and explore the variation rule of its seed vitality index under salt stress in the hope of providing support for the cultivation of salt-tolerant varieties. 【Method】 35 sea-island seeds were used as test materials, and salt stress conditions were simulated by NaCl solution (150 mmol/L) in this experiment. Six indicators of germination potential, germination rate, root length, hypocotyl length, fresh weight and dry weight were statistically analyzed. 【Result】 The results showed that: (1) Under salt stress, the indexes of Gossypium barbadense L. seed were affected differently, among which hypocotyls were most affected and dry weight was the least affected; (2) Compared with the control samples, the maximum, minimum and average of the five indexes of germination potential, germination rate, root length, hypocotyl length and seedling fresh weight were lower than those of the control, while the maximum, minimum and average values of seedling dry weight were higher than those of the control. 【Conclusion】 Cluster analysis results showed that 35 Gossypium barbadense L.seeds were divided into three categories: the first category belonged to high salt tolerance varieties, the second category belonged to medium salt tolerance varieties, and the third category belonged to low salt tolerance varieties.