Abstract:
【Objective】 To ascertain the life history and the change rule of male-female ratio of adult
Hermetia illucens population under indoor feeding conditions, screen and preliminary identify oviposition-inducing bacteria from the egg surface and larval intestinal tract in the hope providing theoretical basis for the large-scale breeding and comprehensive utilization of Hermertia illucens in local area.
【Method】 Hermetia illucen introduced has been reared 4 generations indoors, its life span and life habits in different diets were recorded by direct observation methods, morphological character was observed with microscope, oviposition-inducing bacteria from the egg surface and larval intestinal tract were separated and purified by dilute coating flat plate and flat plate scribing method, and were screened and preliminary identified as the bacteria afterwards.
【Result】 Compared with interior area, the period from eggs to adults was slightly prolonged slightly in Xinjiang, but it was developed well and could be reared for generations in this region. Eggs, larvae, pupae, adults and their genital morphology were observed and recorded. Two strains of bacteria named
e1 and
Y3 with oviposition-inducin effect were screened. The fermentation broth treatment could increase oviposition rate by 6.26 times and 7.40 times respectively compared with the control. The two strains have been tested as Gram-positive bacteria by Gram staining, and preliminary identified as
Bacillus siamensis and
Athrobacter respectively with methods of 16S RNA sequencing.
Y3 was regarded as a potential new bacteria.
【Conclusion】 Annual circular aquaculture of
Hermetia illucens in Xinjiang is feasible.
Hermetia illucens also has the potential of large-scale production and comprehensive development and utilization.