Abstract: 【Objective】 To select the suitable amount of chemical fertilizer in the substrate for pepper(Capsicum frutescence L.)plug seedling cultivation in Hotan region of Xinjiang. 【Method】 In this experiment, hot pepper variety Hangjiao S605 was selected as the testing material, coconut bran∶ fine sand∶ organic fertilizer=20∶1∶1 was used as seedling substrate formula to carry out pepper plug seedling experiments, and during seedling raising, urea, potassium hydrogen phosphate and potassium sulfate were fertilized according to different proportions; By observing and comparing the growth status and physiological indexes of pepper seedlings with different fertilizer application rates in the substrate, the effects of different fertilizer application rates on the growth of pepper plug seedlings were studied so as to screen out the fertilizer application amount suitable for the growth of pepper plug seedlings in Hotan region of Xinjiang. 【Results】 The result showed that when pepper seedlings were raised with the matrix formula of coconut bran∶ commercial organic fertilizer∶ sand = 20∶1∶1(volume ratio), treatment 2 (the application amounts of N,P₂O₅ and K₂O are 200 ,200 , 200 g/m³, respectively) was superior to other treatments in stem diameter, dry weight and dry-fresh ratio. The strong seedling index of treatment 2 was the largest, 0.099,6; The strong seedling index of CK was 0.068,8. Many indexes of treatment 4 (the application amounts of N,P₂O₅ and K₂O are 400, 400,400 g/m³, respectively) were the minimum, and strong seedling index was also the minimum, only 0.0556. 【Conclusion】 Therefore, treatment 2 (the application amounts of N,P₂O₅ and K₂O are 200, 200,200 g/m³, respectively) can be used as the amount of chemical fertilizer in the substrate for pepper plug seedling raising, which is beneficial to the growth of pepper seedlings.