Abstract: 【Objective】 Lilium formolongi Raizan No.1 was used as experimental material, the orthogonal experiment design of three factors and four levels was used to research the effects of different seed pretreatments and cultural temperatures, and cultural method on Lilium formolongi Raizan No.1 seed germination. This experiment also researched the dynamic change of soluble sugars and superoxide dismutase (SOD) in different temperatures. 【Results】 The results showed that the key factor was cultural temperature, followed by pretreatment and cultural method. And as for Raizan No.1, the best way of pretreatment was soaking seed in 4℃ for 7 days, the optimum temperature was 21℃,the optimum culture mode was sowing in soil, its germination was 99.18% and the germination index was 4.65. At different temperatures, the activity of superoxide dismutase (sod) increased first and then decreased at germination stage, but the soluble sugar content showed a tendency of going down first and then up. Among them, the resistance of the seeds under 21℃ treatment and the conversion utilization of the stored substances were higher than those under 15℃,18℃ and 25℃ treatment, which confirmed that 21℃ was the appropriate temperature. 【Conclusion】 The optimum treatment of the Rayshan 1seeds was to sow at 21℃ for 4℃ for 7 days .