Abstract: 【Objective】 To reveal the relationship between photosynthetic characteristics with quality and yield of walnuts. 【Method】 Xinxin 2 and Wen 185 were taken as experimental materials to analyze the relationship between photosynthetic and chlorophyll fluorescence characteristics of the leaves with yield and fruit quality of the two walnut varieties. 【Result】 There was no significant difference in photosynthetic rate and quantum efficiency between Wen 185 and Xinxin 2, which was consistent with the yield and yield per plant of the two varieties. There was a significant difference in the rate of kernel yield between the two varieties. The rate of Wen 185 kernel was significantly higher than that of Xinxin 2, and the respiratory rate was also significantly higher than that of Xinxin 2. The rate of kernel yield may be related to its metabolic characteristics. There was no significant difference in total chlorophyll content between the two varieties, but the chlorophyll a/b and carotenoid content of Wen 185 were significantly higher than those of Xinxin 2. 【Conclusion】 The yields of Wen 185 and Xinxin 2 were related to their photosynthetic characteristics, and their qualities were related to other metabolism. Wen185 may have stronger light protection capability than Xinxin 2.