Abstract: 【Objective】 This study intends to clone the lavender DXS gene and analyze its expression preliminarily in the hope of providing a research basis for revealing the molecular mechanism of this gene in regulating lavender terpenoid synthesis. 【Methods】 Lavender "Zahua" was taken as the testing material to clone lavender DXS gene,and then gene sequence analysis was conducted, expression comparison performed and prokaryotic expression finished. 【Results】 (1)The open reading frame length of lavender DXS gene was 2,181 bp, encoding a protein sequence consisting of 726 amino acids, with an isoelectric point of 6.57 and a molecular weight of about 78.39 KDa. Lavender DXS protein was highly conserved and closely related to the DXS protein of Lavandula angustifolia, Isodon rubescens, and Plectranthus barbatus. (2) The expression level of DXS gene was the highest in the decaying period of Zahua organs and the highest in the blooming period of French blue flower organs. The expression level of DXS gene in the five different developmental stages of Zahua organs was higher than that of French blue. The expression level of DXS gene was the highest in the calyx of Zahua and the highest in the stamens of French blue. The expression level of DXS gene in five different tissues of hybrid flowers was higher than that in French blue (except pistil and stamen). (3) The results of prokaryotic expression analysis showed that the expression of DXS protein was the largest after 4 h induction at 37℃ and IPTG 0.8 mM. 【Conclusion】 There is a positive correlation between DXS gene expression and lavender essential oil production. The results of this study provide material basis and experimental conditions for further confirming the function of DXS gene in the metabolism of lavender terpenoids.