Abstract: 【Objective】 　To explore the expression pattern of ZY96-3 genes related to grain development during grain filling stage. in order to provide reference for further understanding of the molecular mechanism of gene regulation in grain filling. 【Method】 The real-time fluorescence quantitative PCR (qRT-PCR) technique was used to analyze the expression dynamics of 6 genes related to grain development during grain filling stage of ZY96-3. 【Result】 The results showed that the expression patterns of six target genes of ZY96-3 increased first and then decreased, and all of them began to express 7 days after anthesis; TaTGW6 genes were involved in grain weight on the 7 d after flowering to express the highest amount, and the grain size TaCYP78A5 related genes, protein synthesis genes TaPBF - D and starch synthase gene GBSS Ⅰ, SBE1, AGPase1 all around 15 d after flowers express maximum amount; However, the expression level of each gene decreased significantly at 19 d after anthesis. 【Conclusion】 This study showed that the grain-weight related gene TaTGW6 mainly played a key role in the early stage of wheat ZY96-3 grain filling, while the other 5 genes mainly played a role in the middle and late stage of wheat grain filling.