Abstract: 【 Objective 】 To master the molecular epidemiological characteristics of animal Brucellosis between the animals in Xinjiang in the hope of providing scientific basis and technical support for the development of comprehensive prevention and control measures of the disease in Xinjiang. 【 Method 】30 strains were analyzed by AMOS-PCR and MLVA-16 and then comparative analysis was conducted between our results and Brucella from database(http://mlva.u-psud.fr/). After that, a phylogenetic tree was constructed by BioNumerics 6.6. 【 Result 】Among 30 Brucella strains, 3 strains were identified as Brucella burtus, and 26 strains were identified as Brucella melitensis. The result of MLVA-16 showed that 30 Brucella strains were divided into 22 genotypes of 10 different gene groups (A - J group). The prevalent strains had abundant polymorphism in Xinjiang. 【 Conclusion 】Multiple Loci VNTR Analysis (MLVA ) has high discriminability for biotype and difference strains of Brucella. It provides scientific basis for tracing the source of infection and the evolutionary relationship between the epidemic strains.