Abstract: 【 Objective 】 To detect the expression level of WNT2 gene in different tissues of Summerino sheep embryo stage and the molecular structure and evolution characteristics of WNT2 gene in the hope of providing theoretical basis for screening candidate genes related to hair follicles development in this paper.【 Methods 】In this study, the expression of the WNT2 gene in different tissues of the hair follicle of the Merino sheep was studied by qRT-PCR, and the transcription factor of the promoter region of the WNT2 gene was predicted by using the PROMO software, and visualized by the software of Cytscape v3.5.1. The promoter region sequence of WNT2 gene was analyzed by MEGA 7.0 software, and the preference of the codon was analyzed, and the phylogenetic tree was constructed using the DNA sequence of the WNT2 gene of 9 species, such as sheep, goat, cattle and human.【 Results 】The results showed that the expression of WNT2 gene in skin tissue was significantly higher than that in heart, liver, spleen, lung, kidney and muscle tissue. A total of 101 related transcription factors were predicted in the promoter region of sheep WNT2 gene, and four bases were uniformly distributed. CAG and UCU used relatively many codon. The phylogenetic tree analysis of DNA sequence of WNT2 gene showed that the evolutionary relationship between goat and sheep was closer than that of other mammals.【 Conclusion 】 In this experiment, the RT-PCR method of WNT2 gene expression in different tissues of the 135-day embryo stage of Subo Meino sheep was established and its molecular structure and evolution characteristics were analyzed by using bioinformatics software. The internal relationship between the expression of the tissue organ and the WNT2 gene was discussed from the molecular level and the evolutionary character.