Abstract: 【 Objective 】 To investigate the effects of low temperature treatment on the cold resistance of four Hippophae rhamnoides L. varieties and select the varieties with strong cold resistance in the hope of providing a reliable basis for the selection and planting of seabuckthorn cultivars in Xinjiang. 【 Method 】 The yearly branch of four Hippophae rhamnoides L. varieties (Liaojing, Altai News, Chuyi, Alei) in Qinghe County of Xinjiang were used as the testing materials to study the changes of free water/bound water ratio, conductivity, malondialdehyde content, soluble sugar and soluble protein content of Hippophae rhamnoides L. shoots at 10 - -40℃.【 Result 】Under the temperature of -20℃, the free water/bound water ratio, conductivity and malondialdehyde content of the four Hippophae rhamnoides L. branches increased with the decrease of temperature, and the content of soluble sugar and soluble protein decreased, and then increased with temperature. The free water/bound water ratio, conductivity and MDA content of Altai News and Chuyi Hippophae rhamnoides L. branches were relatively low throughout the process of low temperature change. 【 Conclusion 】 Altai News Hippophae rhamnoides L. had the best cold resistance, followed by Chuyi, Liaoyu and Aleyi, indicating Altai News was more suitable for planting in cold regions of Xinjiang.