Abstract: 【 Objective 】 To investigate the dynamic changes of soil enzymes in different intercropping methods in jujube gardens and the effects of water stress on soil enzyme activities in intercropping system. 【 Method 】Two-factor split-zone test design was adopted and two intercropping planting methods were set up in the main area: jujube intercropping cotton and jujube intercropping alfalfa; four water gradients were set in the sub-area: W1: water volume of 3,750 m³/hm² (moderate water stress); W2: water volume of 4,500 m³/hm² (mild water stress); W3: water volume of 5,250 m³/hm² (suitable water); W4: water volume of 6,000 m³/hm² (sufficient water supply). 【 Results 】The results showed that: (1) Under the W2 level, the soil urease activity was the highest. Under the W1 level, the soil sucrase, polyphenol oxidase and catalase activities were the highest. (2) Compared with single cropping, intercropping can significantly increase soil enzyme activity. The soil enzyme activity of intercropping cotton is lower than that of jujube intercropping alfalfa, and jalfalfa intercropping can effectively increase soil enzyme activity. 【 Conclusion 】 Under the level of W1 (3,750 m³/hm²), the intercropping of alfalfa can effectively increase soil enzyme activity.