Abstract: 【Objective】 This study aims to investigate a high-performance liquid chromatograph(HPLC)method for the determination of different kinds of flavonoids and research and analyze the composition and content of flavonoids in pomegranate pericarp of different varieties in Xinjiang in the hope of providing theoretical basis for the breeding and comprehensive development and utilization of pomegranate flavonoids in Xinjiang. 【Method】'Piyaman', 'Qianzihong', 'Saining meng' and'Yechen Pomegranate' were used as the test materials, five kinds of flavonoids: rutin, meletin, quercetin, rhizoma kaempferiae phenol and isorhamnetin were analyzed by HPLC method. The chromatographic separation was performed on Agilent Poroshell 120 EC-C18 (250 mm×4.6 mm,4 μm) using methanol/formic acid/water mixture as the mobile phase at a flow rate of 0.5 mL·min-1through gradient elution, and the detection wavelength was set at 360 nm. 【Result】Results showed that the thorough separation of 5 flavonoids was achieved within 21 min. The method exhibited a linear range of 1-150 μg/mL(R²=0.991,9-0.999,7)and detection limits was 0.2 mg/kg, quantitative limit was 5 mg/kg, the average recovery rates of this method were 76.68%~124.50%. 5 kinds of flavonoids such as rutin, meletin, quercetin, rhizoma kaempferiae phenol and isorhamnetin were detected in all four varieties, among which, quercetin was the highest. 【Conclusion】 There were differences in FLs content between the four different cultivars. 'Piyaman' had the highest total FLs content in peel. From high to low : 'Piyaman'＞'Sainingmeng' ＞'Qianzihong'＞'Yechen Pomegranate'.