Abstract: 【Objective】 Peat was the main substrate for tomato seedling in Hetian area of Xinjiang, and seedling costs were rather high. In this experiment, coconut husk, organic fertilizer and sand in desert were used as main raw materials to prepare tomato seedlings according to different ratios. The purpose of this experiment was to select the best ratio of high quality and strong seedlings of tomato in the hope providing reference for seedling raising of tomato in Hetian area. 【Method】 The tomato variety 'Ouguan' was used as test material and five different treatments and one control were set up with different substrate ratios with the method of raising seedlings with acupoints and plates. All other things being equal, plant height, stem diameter, maximum leaf area, fresh weight, dry weight, root length, root volume, soluble sugar, soluble protein and chlorophyll of tomato seedlings were measured. The effects of different substrate ratio on the growth and physiological and biochemical indexes of tomato seedlings were compared.【Result】All the treatments affected the growth of tomato seedlings. By measuring the growth index and physiological and biochemical indexes of tomato seedlings, it was found that T3 treatment (coconut bran: sand: organic fertilizer =20v: 1v: 1v) had the best effect on tomato seedlings. 【Conclusion】 The ratio of coconut husk: sand: organic fertilizer = 20v: 1v: 1v is beneficial to the growth of tomato hole seedlings, so it is recommended as the substrate formula for tomato seedling in Hetian, Xinjiang.