Abstract: 【Objective】 In order to clarify the received irrigation water and plant nitrogen accumulation and distribution characteristics in one tube serving six rows of drip irrigation and their effects on grain protein content. 【Method】 Seven spring wheat varieties from different regions such as Xinjiang, Inner Mongolia and Ningxia were planted by one tube that serves 6 wheat rows of drip irrigation. Nitrogen accumulation in different parts of plants from the different rows from the burette (the nearest line to the drip tube was R1, the middle line was R2 and the farthest line was denoted as R3) was investigated in the critical periods of the development of wheat: in anthesis and maturity.【Results】Under the condition of one tube that served 6 wheat rows of drip irrigation, during the important stage of plant growth: the jointing-booting stage and booting-flowering stage, the amount of received irrigation water of R3 were 59 and 56 mm, which were similar to the maximum evapotranspiration of 62 and 43 mm in this period. The nitrogen nutrition index at anthesis, nitrogen accumulation, transshipment of nitrogen and grain protein of each organ of the experimental varieties at flowering and mature stages decreased less than the reduced amplitude of the received irrigation water (R2 and R3 decreased by 33.6 and 60.3 relative to R1; The redistribution nitrogen rate, the contribution rate of transport nitrogen to the grain, and the nitrogen harvest index of experimental varieties, R2 and R3 increased in turn with respect to R1. (The rate of nitrogen transport and the contribution rate of transport nitrogen to the grain of Jinqiang No.7 were decreased in this order). The nitrogen accumulation of each organ in anthesis was positively correlated with redistribution nitrogen, and the correlation coefficient was 0.811. The redistribution nitrogen of stem, sheath, leaf and ear were positively correlated with the corresponding nitrogen nutrition index at flowering stage, and the correlation coefficient was 0.403, 0.643 and 0.717, respectively; the nitrogen accumulation of grain was positively correlated with redistribution nitrogen and post-anthesis nitrogen accumulation, and the correlation coefficients were 0.498 and 0.737, respectively. 【Conclusion】In one tube serving six rows of drip irrigation, the key period of crop growth of wheat was not affected by water stress; the nitrogen nutrition index was high, the more nitrogen was transported to grain;the nitrogen of grain was mainly derived from the redistribution nitrogen, the redistribution nitrogen rate, the contribution rate of transport nitrogen to the grain, and the nitrogen harvest index of experimental varieties, R2 and R3 increased in turn with respect to R1.