Abstract: 【Objective】 This paper takes Xinjiang Bashiby sheep with different fur colors(73 red, 53 black and 40 gray) were taken as the research object that polymorphisms of ASIP, TYR, TYRP1, and TYRP2 were detected by PCR-SSCP technique and analyzed. Correlation with the phenotype of the coat. 【Method】Three kinds of coats of Bashiby sheep were selected by random sampling method, and blood was collected with sodium citrate anticoagulant tube.Using the software PIC-CALC and POPGENE 32 to calculate the polymorphic information content and genotype frequency, allele frequency, homozygosity, heterozygosity, effective allele number of ASIP, TYR, TYRP1 and TYRP2 genes in Bashiby sheep, and the distribution of genotypes at this locus was subjected to a chi-square test of Hardy-Weinberg equilibrium.The obtained data was input into an Excel table for sorting, and the difference analysis was performed using SPSS 19.0 software.【Result】 ASIP gene had two genotypes of AA and AB in different coats of Bashibai sheep, and allele B was the dominant allele in the red coat group; Allele A is black, the dominant alleles in the two coat groups of cyan and gray, which was in the Hardy-Weinberg equilibrium state (P>0.05). The red wool polymorphism information content was in a moderate polymorphism, while the black and cyan gray wool polymorphism information content was in a low polymorphism. The TYR gene had two genotypes in the different coat color of the Bashbai sheep group: AA type, AG type and GG type, and the allele G was the three coat color dominant allele TYR gene in Bashbai sheep black and the cyan coat population was in the Hardy-Weinberg equilibrium state(P＞0.05), while the red coat population was in an unbalanced state(P<0.05). There were two genotypes of TYRP1 gene: AA type and GG type, allele A was the dominant allele of red and blue gray coat color; allele G was the dominant allele of black coat group. The TYRP1 gene was in the Hardy-Weinberg equilibrium state in the Bashbai sheep population. The red coat polymorphism information content was highly polymorphic, and the black and cyan gray wool polymorphism information content was in low polymorphism. The PCR-SSCP results of the TYRP2 gene did not show polymorphism. 【Conclusion】 The polymorphisms of ASIP, TYR, TYRP1 and TYRP2 genes are related to the wool color traits of Bashbai sheep, but the specific regulation mechanism needs to be further studied.