Abstract: 【Objective】 To study the inheritance of pepper anthracnose resistance gene, Kaspar marker linked with the anthracnose resistance major gene AnR_GO5 was identified and developed.【Method】Pepper inbred line PBC932 (Capsicum chinense), resistant to anthracnose (Colletotrichum acutatum) was used as the male parent, and the BC₄S₁ and BC₄S₂ populations were obtained by hybridization of the pepper inbred line 77013 (Capsicum annuum) , which was sensitive to anthracnose. The study used Colletotrichum acutatum as the test bacteria, and the green ripe fruit was inoculated by microinjection method, and the phenotypic analysis was carried out according to the diameter of the lesion. Based on the results of re-sequencing of anti- and susceptible parents, Kaspar markers linked to anthracnose in green ripening stage were developed to construct a genetic linkage map.【Result】The diameter of lesions on the surface of pepper fruit was continuous and accorded with the genetic characteristics of quantitative traits. The anthracnose resistance gene AnR_GO5 was mapped to the marker P5L-866 of the 5th linkage group and the marker P5L-259 by linkage analysis, and the genetic distance was 2.9 cM. The marker P5L-117 developed was closely linked to the gene and the labeling accuracy was 93.5%.【Conclusion】 In the BC₃S₁ population of pepper, the fruit green ripening resistance gene was located in the marker interval of chromosome 5. Pepper anthracnose resistance is dominantly hereditary and is controlled by two pairs of major genes.