Abstract: 【Objective】 In this study, D-101 macroporous adsorption resin was used to separate and purify total flavonods from fruits of pomegranate cultivars from Xinjiang.【Method】 Based on the single factor study, the conditions for the separation and purification of total flavonoids from pomegranate skin by DX101 macroporous adsorption resin were further studied by investigating the temperature, pH, adsorption penetration curve, eluent concentration and ratio of material to liquid, and the optimum technological conditions were found.【Result】The optimal purification conditions were as follows:pH 3-4,temperature of 30℃, material ratio of 1∶4(g/mL), the volume of ethanol in the eluent was 90%. Under this process condition, the purity of total flavonoids in pomegranate peel increased from 7.25% to 17.32%. 【Conclusion】Under this process condition, the non-target ingredients, such as pigment and chlorophyll can be eluted effectively, and the total flavonoids of pomegranate cultivars of Xinjiang can be separated and purified scientifically and reasonably and the operation is simple, safe and of low cost.