Abstract: 【Objective】 To analyze the expression pattern of Haloxylon ammodendron 14-3-3 protein gene HaFT-9 under different stresses, and the binding characteristics of HaFT-9 protein, so as to provide a theoretical basis for studying the molecular mechanism of stress resistance in Haloxylon ammodendron.【Method】A 14-3-3 protein gene, HaFT-9, was cloned using Haloxylon ammodendron cDNA as a template, and the expression pattern of this gene under different stresses was analyzed by qRT-PCR, and the HaFT-9 protein binding properties were verified by yeast two-hybrid assay.【Result】The gene for the Haloxylon ammodendron 14-3-3 protein with an ORF length of 789 bp was cloned and named HaFT-9. Under 20% PEG6000, 200 mmol/L NaCl, and 100 μmol/L ABA treatment, the expression of HaFT-9 all peaked at 6 h of stress, and under 20 μmol/L IAA treatment, the expression of HaFT-9 peaked at 1 h of stress. HaFT was up-regulated in the normal temperature recovery stage in the adaptation experiment of high temperature stress by simulating surface high temperature stress at 40℃, 55℃and 55℃, and 65℃. Yeast two-hybrid experiment showed that HaFT-9 could form homologous dimer with itself.【Conclusion】The HaFT-9 gene (accession number API85525.1) was cloned, which could respond to drought stress, salt stress, ABA and IAA treatment, and was associated with adaptability to high temperature stress. HaFT-9 protein can interact with itself to form homodimer.