Abstract: 【Objective】 The projiect aims to investigate the effects of different doses of allicin supplementation on physiological, biochemical and immune indexes of Yili horse after exercise, which might provide theoretical guidance for healthy breeding of sports Yili horse. 【Method】 After the test, 16 Yili horses were fed with allicin in four groups for 30 days, on the basis of supplemental feeding for 0 days, and then sampled once every 10 days, four times before and after the race. The period of each sampling was immediately after the race. The venous blood samples were collected to measure the related indexes, and the results were analyzed by double factor variance analysis. 【Result】 After 10 days of feeding, the lactate value was significantly lower than that of the basic value (P<0.05). After 20 days of supplementary feeding, the potassium ion was significantly lower than that of the basic value (P<0.05). After 30 days of supplementary feeding, the calcium ion was significantly lower than that of the base value (P<0.05). About the CK, the horses with daily supplement of 100 mg/kg allicin was significantly lower than that of the control group (P<0.05).The C₄ of horses with 300 mg/kg was extremely significantly higher than that with 100 mg/kg (P<0.01). About the IgM, the horses with 500 mg/kg was extremely significantly higher than the control group (P<0.01). 【Conclusion】 The effect of controlling the amount of allicin between 300 mg/kg and 500 mg/kg on the exercise horse Is obvious, and it can effectively improve the immunity of the body, and in terms of enhancing the body's immunity, the amount of allicin supplemented is more effective than the Feeding time.