Abstract: 【Objective】 This study aims to elucidate the major pathogenic bacteria and their drug resistance that caused a large number of deaths for explained reasons in a rabbit cooperative in the Shihezi region. 【Method】 In this study, routine bacterial isolation and culture, morphological observation, biochemical tests, PCR identification and capsular serotyping, pathogenicity test, drug susceptibility test were used to identify the main pathogens and their biological characteristics. 【Result】 The results showed that the main cause of the disease was capsular serum type A Pasteurella multocida, which had strong pathogenicity with mixed infection of Escherichia coli. Of the 30 commonly used clinical antibacterials, the disease was sensitive to the third generation cephalosporins such as cefoperazone, cefotaxime, ceftriaxone, ceftazidime, cefepime, some quinolone antibiotics such as ofloxacin, levofloxacin and florfenicol, and was moderately sensitive or resistant to more than 20 other drugs and the isolated strains showed different degrees of multidrug resistance. 【Conclusion】 The capsular serum type A Pasteurella is the main pathogen causing large-scale death of rabbits in Shihezi area, and it is highly resistant to common antibiotics in clinical practice. It enriches the research foundation of Pasteurella in causing various animal diseases in this area. Provide clinical evidence for drug use and prevention and control.