Abstract: 【Objective】 A novel PsaH gene was cloned from suaeda salsa,which was a protein subunit of photosystem I. 【Method】 This project aimed to study the structure and function of PsaH gene, the full-length cDNA sequence of PsaH gene was obtained from Suaeda salsa salt-stress cDNA library through sequencing, and it was validated by bioinformatics analysis, and named SsPsaH (GeneBank Accession Number:KC4048847). 【Result】 The full-length of SsPsaH gene was 770 bp, containing 438 bp open reda frame (ORF), and encoding 145 amino acids residues. Based on the predicted analysis of bioinformatics, the SsPsaH was located in chloroplast membrane with transmembrane region, and it was a hydrophilic and unstable protein without signal peptide. Its secondary structure elements were mainly random coil. The SsPsaH gene was highly conservative in different plant species. Phylogenetic tree analysis showed that the protein was closely related to the PsaH protein of Spinacia oleracea. The results of RT-PCR indicated that SsPsaH gene was expressed in root, stem and leaf, but it was higher in stem and leaf than in root. This study has provided a basis for the further study on the salt-tolerance function of the gene and its role in the mechanism of salt tolerance in halophytes, and provided a candidate gene for improving the molecular breeding of salt-tolerance in crops. 【Conclusion】 SsPsaH gene is a protein subunit H of photosystem I. This study would lay the foundation for future studying on the gene function and the role in salt-tolerance of Suaeda salsa.