Abstract: 【Objective】 The purpose is to identify genes in response to Verticillium dahlia by the virus induced gene silencing (VIGS). 【Method】 With the construction of VIGS virus (virus induced gene silencing) vector by molecular way, we established an Agrobacterium-mediated virus-induced gene silencing (VIGS) assay in cotton. To study function of GhCPK gene family, a GhCPK gene was silenced in cotton using VIGS assay, then the disease index of the gene-silenced cotton seedlings were investigated when they were challenged with Verticillium dahlia. 【Result】 By infecting cotton cytoledons with VIGS virus vector containing GhCPK fragment, the GhCPK fragment is identified and catalyzed into dsRNAs.The combination of dsRNAs with other RNA produce RNA induced silencing comlex (RISC) which can target and degrade endogenous GhCPK mRNA and lead to the GhCPK gene silenced.As the result of silencing GhCPK gene in cotton, the GhCPK silenced cottons showed enhanced sensitivity to Verticillium dahlia, which suggested that GhCPK gene may play a role in regulating disease resitance in cotton. 【Conclusion】 The virus induced gene silencing technology is an efficient way for identifyingGhCPKs gene in response to disease stress in cotton.