Abstract: 【Objective】 This project performed aims to study the effect of non-starch polysaccharide enzyme (NSP Enzyme) on growth and nutrient metabolic rate in 23 weeks Hyline brown hens. 【Method】 One thousand and six hundred hyline brown hens aged 23 weeks were randomly divided into 4 experimental groups with 10 replicates in each group, 16 chickens in each replicate. The positive control group (group PC) was fed basic diet with normal energy level; The negative control group (NC group) was fed a diet tin which the metabolizable energy (ME) level of 628 KJ/kg was reduced in comparison with the PC group diet; The compound non-starch polysaccharide enzyme l and the compound non-starch polysaccharide enzyme 2 were added to the diet of NC group, respectively. 【Result】 The results showed that in comparison with the negative control group,.the addition of non-starch polysaccharide in corn soybean miscellaneous diets could reduce hyline brown hens feed intake and feed egg ratio, and improve the nutrient metabolic rate, but the difference was not significant, which had no effect on egg weight . 【Conclusion】 The addition of non-starch polysaccharides enzyme to the corn soybean meal miscellaneous meal diet can replace part of the oil (628 KJ/kg AME energy) level, and does not affect the production performance of laying hens.