Abstract: 【Objective】 Cordyceps militaris contains many kinds of active substances such as nucleosides, polysaccharides, cordycepin, sterols, sugar alcohols, enzymes, pigments and other active substances, among which cordycepin, adenosine, polysaccharide and so on are widely studied. 【Method】 The extraction of cordycepin and adenosine was commonly used by ultrasonic method, reflux method, percolation extraction method and so on. In this paper, accelerated solvent extraction was used to obtain the active substances of cordycepin and adenosine from the fruiting bodies of Cordyceps militaris. The optimum conditions were obtained by the method of orthogonal experiment and the rapid extraction method. The method of accelerated solvent extraction was optimized by orthogonal experiment. In addition, four factors (temperature, static extraction time, ethanol concentration and cycle number) were designed to determine the optimal combination conditions. 【Result】 The optimum combination conditions for the extraction of cordycepin and adenosine by accelerated extraction were obtained by experiments. 【Conclusion】 The optimum conditions for the extraction of adenosine by accelerated extraction were as follows: temperature 70℃, time 5 min, ethanol concentration 20% and cycle times 2, and the optimum conditions for extracting adenosine were as follows: temperature 100℃, time 10 min, ethanol content 0 and cycle times 2 times.